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. 2016 Aug 4;113(34):E4956–E4965. doi: 10.1073/pnas.1525082113

Fig. 2.

Fig. 2.

Overall folding of WT P4-P6 and constructs with the native P5c secondary structure enforced. (A) The local equilibrium between the native and alternative P5c secondary structure of WT P4-P6 and constructs with the native P5c secondary structure enforced by two (Nat+2) or three (Nat+3) base pairs (46, 50, 77, 82). (B) Scatter plot of folding versus unfolding rate constants for WT and Nat+3 P4-P6. Data for 100 randomly selected molecules with the median folding and unfolding rate constants denoted by red circles (Dataset S1). See SI Appendix, Fig. S1 for data for a variant with the alternative P5c secondary structure (Alt+2). (C and D) Comparison of the folding rate (C) and equilibrium (D) constants of WT, Nat+2, and Nat+3. Error bars correspond to the bootstrap-estimated 95% confidence intervals (SD = 2σbootstrap). See SI Appendix, Table S1 for data and SI Appendix, Supporting Methods for experimental details. Conditions were as follows: 50 mM MOPS, pH 8.0, 5 mM MgCl2, and 100 mM KCl at 23 °C. (E) Overall P4-P6 folding occurs from an unfolded state with native P5c secondary structure (MU).