Fig. 3.

Vector correction to determine WT dUMP₁ peak positions. The two schematic diagrams show how CSPs were calculated for each dUMP binding event (SI Materials and Methods). (A) The CSPs for binding the first dUMP, CSP₁, were calculated directly from the apo and dUMP₁ peak positions of the two mixed labeled dimer (MD) samples (thick arrows). Dashed arrows show the CSP due to the mutation (mut). (B) CSPs for binding the second dUMP, CSP₂, were calculated as the vectors connecting the WT dUMP₂ peak (in blue) and the dUMP₁ peaks for WT (empty red and green circles), which were reconstructed by applying the CSP₁ vectors to the apo WT peak (dashed arrows).