Fig. 5.

Line plots of ¹H chemical shifts. These line plots show how the chemical shift behaviors observed from ligand state multiplets are distributed throughout the protein. ¹H chemical shift changes (relative to apo) are plotted for apo (black), dUMP₂ (blue), dUMP₁^bound (red), and dUMP₁^empty (green). Each of the dUMP₁ points is labeled with the distance (in Å) of that residue (red and green spheres) from the bound dUMP (sticks). Distances are measured from the amide N to the centroid of the bound dUMP. (A) Behaviors of residues at opposite ends of the dimer interface. (B) Behaviors of interior residues. (C) Behaviors of residues at the center of the dimer interface.