Fig. 6.

Observable patterns for ligand state multiplets. Schematic HSQC peak patterns according to allosteric model (A and B) or observed for dUMP binding in TS as resolved triplets (C–F) or quartets (G and H). Peaks are shown for apo/lig₀ (black), lig₁^bound (red), lig₁^empty (green), and lig₂ (blue). In C, two partial shifting behaviors are shown in which lig₁ partial peak shifting is observed in the binding subunit (light red) or in the empty subunit (dark green). Behaviors are observed for supershifting (F), orthogonal shifting (G), and reverse shifting (H). In each panel, the percentages indicate the abundance of the peak pattern for dUMP binding to TS.