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Cross-validation of the mixed dimer strategy using a singly bound diligand. Chemical shift changes measured for the empty subunit of a mixed dimer bound with diligand were compared with those for the empty subunit of WT TS bound with diligand (1 molar equivalent of diligand per TS dimer). Correlations for (A) 1H chemical shifts, (B) 15N chemical shifts, and (C) 1H-15N CSPs are shown with outliers marked with an asterisk. The line represents a correlation coefficient of 1.
