Fig. S3.

Supershifted residues in the dUMP₁ and diligand₁ states. Residues that exhibit supershifting upon binding the first dUMP (Top) or diligand (Bottom) are shown, using the same hinge rotation as in Fig. 2. Supershifted residues, shown as red spheres, have a CSP greater than 0.025 ppm with a supershift greater than 0.01 ppm. dUMP (A) and diligand (C) are shown in blue, and unassigned residues are shown in yellow. (A) Supershifted residues in the dUMP₁^bound subunit. (B) Supershifted residues in the dUMP₁^empty subunit. (C) Supershifted residues in the diligand₁^bound subunit. (D) Supershifted residues in the diligand₁^empty subunit.