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. 2016 Aug 9;113(34):9581–9586. doi: 10.1073/pnas.1604277113

Fig. 6.

Fig. 6.

RNF122 promotes proteasomal degradation of RIG-I protein by mediating K48-linked ubiquitination via the residue K115 and K146 of RIG-I. (A) HEK293T cells were transfected with RIG-I plasmid, along with increasing amounts of plasmid expressing RNF122. At 24 h after transfection, the expression of Myc, V5, and β-actin were determined by Western blot. (B) HEK293T cells were transfected with the indicated plasmids for 24 h and treated with MG132 for 8 h. Cell lysates were immunoblotted with the indicated antibodies. (C, D, G, and H) HEK293T cells were transfected with the indicated plasmids. After 24 h, cells were treated with MG132 for 8 h and/or infected with VSV for 4 h. Cell lysates were immunoprecipitated using anti-Myc antibody, followed by Western blot analysis using the indicated antibodies. (E, F, and I) HEK293T cells were analyzed 24 h after transfected with the indicated plasmids together with an Ifnb promoter-driven reporter plasmid, and the luciferase activity was determined. Data are from three independent experiments (mean ± SEM; *P < 0.05 and **P < 0.01).