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. 2016 Aug 8;113(34):9509–9514. doi: 10.1073/pnas.1610403113

Fig. S2.

Fig. S2.

Isolation of proteins associated with Xkr8 and the Xkr8 complex. (A) Isolation of the Xkr8-associated proteins. The light membrane fractions from PLB or PLB-hXkr8 were solubilized with CL47 and applied to anti-Flag beads. Proteins were eluted with CL47 containing 1% Triton X-100 (asterisk), and then with Flag peptide, separated by SDS/PAGE and silver-stained. (B) Isolation of the Xkr8 complex. Light membrane fractions from PLB expressing the Flag-tagged hXkr8 were lysed in LMNG and loaded onto anti-Flag beads. The hXkr8 complex was eluted with Flag peptide, separated by BN-PAGE, and stained with CBB. The broad band at 240 kDa (arrowhead) was analyzed by mass spectrometry (table).