Fig. S3.

Structure of human basigin and neuroplastin, and its editing by CRISPR-Cas system. (A) The structure of human basigin (hBSG) and neuroplastin (hNPTN) is schematically shown. Ig, Ig-like domain; T, transmembrane. (B) CRISPR-Cas–mediated editing of the hBSG and hNPTN genes. The hBSG and hNPTN target sequences (upper line) and the sequences of the mutated alleles (lower lines) in DKO-hXkr8 cells are shown. The protospacer sequence is in light blue, and the protospacer-adjacent motif (PAM) is in green. The number of added or deleted nucleotides is shown at the right. In one allele of BSG gene, 48 nucleotides including the splice acceptor site (underlined) were deleted.