Table 1. BALF cell number and distribution at 24 h post-exposure after repeated i.t. instillations of carbon black (CB) and/or lipopolysaccharide (LPS) in ApoE-/- mice.
| Group | Vehicle | Low-dose CB | High-dose CB | LPS | Low-dose CB+LPS | High-dose CB+LPS |
|---|---|---|---|---|---|---|
| Study 1 | ||||||
| Total cells (x 103) | 187.2 ± 32.1 | 378.9 ± 39.7*** | 243.5 ± 37.7 | 405.0 ± 44.5*## | 358.5 ± 39.0## | 297.1 ± 30.2*## |
| Neutrophils (x 103) | 8.5 ± 5.2 | 85.2 ± 16.5*** | 52.1 ± 11.5*** | 261.0 ± 25.8## | 164.5 ± 32.9*##$ | 196.5 ± 26.1*##$ |
| Macrophage (x 103) | 98.1 ± 15.3 | 138.4 ± 10.2* | 134.9 ± 13.4* | 108.0 ± 13.2## | 130.7 ± 14.7## | 86.7 ± 12.9 |
| Eosinophils (x 103) | 60.1 ± 20.7 | 87.2 ± 24.1 | 26.3 ± 15.7 | 20.0 ± 8.9 | 32.7 ± 7.8 | 5.4 ± 3.0## |
| Lymphocytes (x 103) | 3.2 ± 1.1 | 53.5 ± 9.3*** | 20.7 ± 7.3*** | 10.0 ± 2.4 | 20.3 ± 7.2 | 4.0 ± 0.9 |
| Epithelial (x 103) | 17.1 ± 4.1 | 14.6 ± 3.8 | 9.4 ± 1.7 | 6.1 ± 2.7## | 10.3 ± 2.1 | 4.5 ± 1.4## |
| Study 2 | ||||||
| Total cells (x 103) | 103.3 ± 17.4 | 104.8 ± 16.7 | ND | 294.4 ± 27.3*** | ND | ND |
| Neutrophils (x 103) | 1.6 ± 0.6 | 11.6 ± 4.4*** | ND | 176.7 ± 23.3*** | ND | ND |
| Macrophage (x 103) | 72.3 ± 12.1 | 66.2 ± 7.1 | ND | 98.1 ± 8.8 | ND | ND |
| Eosinophils (x 103) | 14.7 ± 5.2 | 14.2 ± 7.9 | ND | 9.8 ± 3.3 | ND | ND |
| Lymphocytes (x 103) | 1.8 ± 0.9 | 2.6 ± 0.8 | ND | 2.1 ± 0.8 | ND | ND |
| Epithelial (x 103) | 13.0 ± 2.4 | 10.1 ± 2.1 | ND | 7.8 ± 2.1 | ND | ND |
* P<0.05
*** P<0.001 in CB exposed group compared to vehicle group.
## P<0.01 in LPS group compared to vehicle control group.
$ P<0.05 in CB+LPS exposed group compared to LPS group.
Data is presented as mean ± SEM. The BALF cells were not determined (ND) in all groups in study 2.