Skip to main content
. 2016 Aug 29;11(8):e0162023. doi: 10.1371/journal.pone.0162023

Fig 4. Absence of astrocyte specificity for the marmoset- and human-derived GFAP promoters in the mouse cerebrum.

Fig 4

(A-C) Cerebral slices lentivirally expressing GFP under the control of the 0.3-kb marmoset-derived cjGFAP (A), 0.3-kb mouse-derived mGFAP (B) or 0.3-kb human-derived hGFAP (C) promoter were triple-immunostained for GFP (green), GFAP (magenta) and NeuN (a neuronal marker, cyan). Note the predominant expression of GFP in neurons (arrow) by the marmoset- and human-derived promoter, which is in sharp contrast to the astrocyte-specific expression (arrowhead) by the mouse-derived promoter. Scale bars, 50 μm. (D) Schema depicting morphology of neuron and astrocyte in the cerebral cortex. (E) Quantitative analysis of the astrocyte specificity for the cjGFAP, mGFAP and hGFAP promoters. More than 300 GFP-positive cells from 3 mice (3 slices/mouse) were randomly selected, and the ratio of GFAP-labeled astrocytes were determined in these random selections. Asterisks indicate statistically significant differences between the mouse promoter and the marmoset or human promoter, as determined by one-way ANOVA followed by Tukey’s post hoc test, ***p<0.001.