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. Author manuscript; available in PMC: 2017 May 1.
Published in final edited form as: J Struct Biol. 2016 Feb 12;194(2):180–190. doi: 10.1016/j.jsb.2016.02.015

Figure 3. Reduced and oxidized EhTrxR.

Figure 3

A, ESI-MS analysis of reduced (red) and oxidized (blue) EhTrxR. 2 Da experimental mass difference between NADPH-plus and NADPH-minus samples corresponds to reduction of one disulfide bond in the NADPH-treated sample. B, 2Fo-Fc electron density map (blue mesh) contoured at 1.0 σ shows the oxidized redox-active sulfhydryl groups forming a 4-residue disulfide loop.