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. Author manuscript; available in PMC: 2017 Aug 25.
Published in final edited form as: Cell. 2016 Aug 25;166(5):1257–1268.e12. doi: 10.1016/j.cell.2016.07.044

Figure 7. Effects of BI-B2 on HIV-1 IN interactions with viral RNA in virions.

Figure 7

(A) Virions from WT HIV-1NL4-3 and the escape mutant HIV-1NL4-3 IN(A128T) were prepared in the presence and absence of 10 μM BI-B2 in virus producer cells and used to infect target cells to determine relative infectivity. The average values of three independent experiments and corresponding standard deviations are shown. (B) Autoradiograms of IN-RNA and NC-RNA crosslinks adducts from virions of WT HIV-1NL4-3 and HIV-1NL4-3 IN(A128T) prepared in the presence and absence of 10 μM BI-B2 (upper two panels). Corresponding western blot analysis of IN and NC in immunoprecipitated fractions (lower two panels). (C) CLIP-seq analysis of IN binding to viral RNA genome from virions of WT HIV-1NL4-3 (upper panel), WT HIV-1NL4-3 + BI-B2 (middle panels), and HIV-1NL4-3 IN(A128T) + BI-B2 (lower panel).