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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Endocrinol. 2016 Oct;231(1):35–48. doi: 10.1530/JOE-16-0229

Fig. 5. IL-33 treatment restored the fraction of ILC2/eosinophil pathway and UCP1 expression in WAT of HFD-fed mice.

Fig. 5

(A) The mRNA levels of IL-33 in inguinal fat were induced by cold exposure but suppressed by HFD feeding and leptin deficiency. The circulating levels (B) and protein level (C) in sWAT of IL-33 were significantly increased by cold stress, while decreased by HFD feeding and leptin deficiency. The level of IL-33 was measured with ELISA analysis. The percentages of ILC2s and eosinophils in CD45+ cells from gate R1 and gate R2 respectively in sWAT (D) and in eWAT (E) were significantly increased by IL-33 injection. (F) The protein levels of UCP1 in sWAT and eWAT were upregulated by IL-33 administration in HFD-fed mice. The expression levels of TH and phosphorylation of PKA substrates in sWAT (G) and in eWAT (H) were upregulated by IL-33 administration in HFD-fed mice. Tubulin was used as the loading control. The data in Fig. 5A–5F were presented as the mean ± SEM. *p<0.05, **p<0.01.