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. Author manuscript; available in PMC: 2017 Aug 25.
Published in final edited form as: Cell. 2016 Aug 25;166(5):1308–1323.e30. doi: 10.1016/j.cell.2016.07.054

Figure 2. Validation of markers for six BC types.

Figure 2

(A) Representative markers (columns) enriched in BC clusters (rows) predicted and validated in this study. Representation as in Figure 1F.

(B–D) Validation of RBC-specific genes Vstm2b, Casp7, and Rpa1 by FISH combined with PKCα immunostaining, which marks RBCs.

(E–I) Validation of new markers of BC3A, BC3B, BC4, BC6, and BC7 against cell morphology. Leftmost panels show representative drawings of these types based on EM reconstructions (Helmstaedter et al., 2013), middle panels show lentiviral labeling of single BCs combined with FISH for the indicated gene in retinal cross sections. Dashed lines are drawn from calretinin antibody labeling within sublaminae (S) 2, 3, and 4. Insets show localization of FISH signal within virus-labeled cell bodies. Rightmost panels show FISH labeling of cell bodies on retinal whole mounts. To reduce background puncta in the GFP+ lentivirus labeled cells, an outlier removal noise filter was applied (Methods and Resources). Scale bars indicate 20 μm for main panels and 10 μm for insets.

See also Figures S4–S5 and Tables S3–S4.