Table 2.
Pdu mutants | Doubling time (h) |
---|---|
WT/pLac22 | 19.4 ± 0.8 |
ΔpduA/pLac22 | 10.5 ± 0.8* |
ΔpduA/pLac22-pduA (induced with 500 μM IPTG) | 14.2 ± 0.3* |
ΔpduJ/pLac22 | 10.1 ± 0.7* |
ΔpduJ/pLac22-pduJ (induced with 50 μM IPTG) | 18.1 ± 0.5 |
ΔpduA/pLac22-pduJ (induced with 500 μM IPTG) | 9.5 ± 1.2* |
ΔpduJ/pLac22-pduA (induced with 50 μM IPTG) | 17.1± 1.1 |
ΔpduA/pLac22-pduA (uninduced) | 9.5 ± 0.7* |
ΔpduJ/pLac22-pduJ (uninduced) | 8.8 ± 1.1* |
Doubling times were calculated from at least three biological replicates measured in triplicate. The error estimate shown is ± one standard deviation
p-value <0.001 compared to wild-type.