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Effect of complementation on DDH activity
| MCPs from Pdu Mutants | Specific Activity (μmol min−1mg−1) |
|---|---|
| WT/pLac22 | 27.3 ± 0.8 |
| ΔpduJ/pLac22 | 33.7 ± 0.7 |
| ΔpduJ/pLac22-pduJ | 27.8 ± 0.7 |
| ΔpduA/pLac22-pduA | 29.5 ± 0.8* |
| ΔpduA/pLac22 | 34.8 ± 1.1 |
| ΔpduA/pLac22-pduJ | 34.5 ± 0.9* |
| ΔpduJ/pLac22-pduA | 28.7 ± 0.7 |
The DDH activities are based on at least three biological replicates measured in triplicate. The error estimate shown is ± one standard deviation.
p-value <0.001 compared to wild-type/pLac22.
