Table 2.
One-step, asymmetric RT-amplification microarray detection of West Nile virus and enterovirus RNA in single-plex reactions. Data are the average signal to noise ratios from n = 3 technical replicates. Bold type indicates specific amplification microarray signals. No template controls were all negative (not shown).
| Target (gene copies per reaction) | ||||||
|---|---|---|---|---|---|---|
| Probe Specificity | Enterovirus RNA | West Nile virus RNA | ||||
| 104 | 103 | 102 | 104 | 103 | 102 | |
| HSV1 | 0.21 | 0.05 | 0.09 | 0.18 | 0.39 | 0.12 |
| HSV2 | −0.25 | 0.08 | −0.08 | −0.46 | −0.20 | −0.30 |
| VZV | 0.76 | 0.38 | 0.39 | 0.20 | 0.07 | −0.01 |
| GFP | −0.25 | 0.25 | 0.01 | 0.13 | −0.27 | 0.07 |
| EVU | 405.00 | 198.73 | 29.57 | −0.16 | 0.08 | −0.44 |
| CMV | 3.49 | 1.38 | 0.74 | 0.13 | 0.15 | 0.11 |
| HHV6 | 0.03 | 0.57 | 0.11 | 0.27 | 0.22 | −0.35 |
| WNV | −0.26 | 0.03 | 0.01 | 1,093.61 | 824.32 | 1,263.02 |