Figure 2.

Microarrays for differential protein displays. Proteins from controls and samples are isolated and conjugated to different fluorescent molecules, for example Cy3 and Cy5. The samples are mixed in equal amounts and incubated simultaneously on an antibody microarray. Then, target molecules will be captured by their specific antibody and the differences in protein expression are directly reflected by the overlay of the color signal. The section marked with a yellow circle reflects the difficulty of quantification because a prominent signal could be the result of a single protein, but also of a large protein complex.