Table 2.

List of capture agents used in protein arrays, source and technique.

Capture agent	Source of proteins	Technique
Mab *	mouse	Hybridoma
sc Fv */Fab * diabodies	antibody libraries	Phage display, in vitro evolution
Affinity binding agents	recombinant fibronectin structures	In vitro evolution
Affibodies
Aptamers (DNA, RNA, peptide)
Receptors ligands	synthetic	Combinatorial chemistry
Substrates of enzymes	synthetic; pro-and eukaryotic organisms	Protein purification, recombinant protein technology(bacterial, fusion proteins, baculovirus, peptide synthesis)

* Abbreviations: Fab, antigen-binding fragment; sc Fv, single-chain variable region fragment; Mab, monoclonal antibody.