Table 2.
Slide- and well-based surfaces evaluated as solid supports for antibody microarrays.
| Format | Name | Surface Chemistry | Surface Geometry a | Binding Chemistry | Supplier |
|---|---|---|---|---|---|
| Slides | Nexterion H | NHSb polymer | 3D | Covalent | Schott |
| Nexterion P | Hydrophilic NHS polymer | 3D | Covalent | Schott | |
| GAPS II | Aminopropylsilane | 2D | Ionic | Corning | |
| NHS glass | NHS glass | 3D | Covalent | PolyAn | |
| NHS polymer | NHS polymer | 3D | Covalent | PolyAn | |
| Epoxy glass | Epoxy glass | 3D | Covalent | PolyAn | |
| Epoxy polymer | Epoxy polymer | 3D | Covalent | PolyAn | |
| FAST | Nitrocellulose | 3D | Adsorption | Whatman | |
| SuperProtein | Hydrophobic polymer | 2D | Adsorption | Arrayit | |
| Silane-Prep | Aminoalkylsilane glass | 2D | Ionic | Sigma | |
| Black MaxiSorp | Hydrophilic polymer | 2D | Adsorption | NUNC | |
| Clear MaxiSorp* | Hydrophilic polymer | 2D | Adsorption | NUNC | |
| 96-well plates | Clear MaxiSorp | Hydrophilic polymer | 2D | Adsorption | NUNC |
| SciPLEXPLATE | Polystyrene | 2D | Adsorption | Scienion | |
| Costar | Polystyrene | 2D | Adsorption | Corning |
a 3D—three-dimensional, 2D—two-dimensional; b N-Hydroxysuccinimide; * This slide was only used as a reference when evaluating plate-based surfaces.