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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Cell Biochem. 2015 Oct;116(10):2354–2364. doi: 10.1002/jcb.25186

Figure 1. Expression of I-Smads and TNF-a in aortic tissues of normolipemic and hyperlipidemic mice.

Figure 1

(A) Serum cholesterol levels of C57BL/6 and Ldlr−/− mice. (B) Realtime RT-qPCR analysis of aortic tissues from C57BL/6 and Ldlr−/− mice for Smad6, Smad7, and TNF-a expression. *p < 0.05 vs. respective C57BL/6 controls. (C) Left - Immunofluorescence images of aortic valve leaflets (white arrow) and aorta (yellow arrow) from C57BL/6 (n = 7) and Ldlr−/− (n= 7) mice for Smad6. Magnification: 10X. Right - Quantitation of the images (normalized per tissue area) using image analysis software (Metamorph Advanced v7.7). *p < 0.05 vs. respective C57BL/6 controls. (D) Left - Immunofluorescence images of aortic valve leaflets (white arrow) from C57BL/6 (n = 6) and Ldlr−/− (n= 6) mice for Smad7. Right - Quantitation of the images (normalized per tissue area) using the image analysis software. (E) Left - Immunofluorescence images of aortic valve leaflets from C57BL/6 (n = 7) and Ldlr−/− (n= 7) mice for TNF-a. Right - Quantitation of the images (normalized per tissue area) using the image analysis software. *p < 0.05 vs. respective C57BL/6 controls.