Skip to main content
. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: J Cell Biochem. 2015 Oct;116(10):2354–2364. doi: 10.1002/jcb.25186

Figure 6. Effects of Smad6 knockdown on TNF-a-induced oxidative stress and Wnt signaling in mVIC.

Figure 6

(A) Realtime RT-qPCR analysis of Smad6 expression in response to hTNF-a (25 ng/ml), Trolox (400 μM) or PDTC (10 μM), as indicated, for 10 days. Cells were cotreated with PDTC or pretreated with trolox for 1 hr prior to TNF-a addition at each media change. *p < 0.05 vs. vehicle control (B) Realtime RT-qPCR analysis of hemeoxygenase-1 (HO-1) and catalase expression in response to hTNF-a (25 ng/ml) for 10 days. *p < 0.05 vs. respective vehicle control; **p < 0.05 vs. ct shRNA hTNF-a. (C) Realtime RT-qPCR analysis of Wnt downstream targets Axin-2 and LEF-1 expression in response to hTNF-a (25 ng/ml) for 10 days. *p < 0.05 vs. respective vehicle control; **p < 0.05 vs. ct shRNA hTNF-a.