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. Author manuscript; available in PMC: 2016 Aug 31.
Published in final edited form as: Cell Rep. 2016 Jul 21;16(5):1326–1338. doi: 10.1016/j.celrep.2016.06.076

Figure 2. CHIKV 181/25 Persists in Rag1−/− Mice.

Figure 2

(A–D) WT or Rag1−/− mice were inoculated with 1,000 PFU of AF15561 or 181/25. At (A) 3 dpi of Rag1−/− mice or (B) 28 dpi of WT or Rag1−/− mice, viral RNA in the right ankle was quantified by RT-qPCR. Infectious virus in the (C) serum or (D) right ankle was quantified by plaque assay.

(E) WT mice were inoculated in the left rear footpad with clarified ankle tissue homogenate containing 6 PFU of virus from (D) AF15561- or 181/25-infected animals or 6 PFU of CHIKV strain AF15561. At 28 dpi, CHIKV RNA in the right ankle was quantified by RT-qPCR. Horizontal bars indicate mean values. Dashed lines indicate the limit of detection. Data are from two or more independent experiments. P values were determined by Mann-Whitney test (A and D) or one-way ANOVA with a Tukey’s multiple comparison test (B, C, and E). *, P < 0.05, ***, P < 0.001, ****, P < 0.0001.