Fig (5). Gel electrophoresis for TNF-α mRNA.

Fig (5): (a) lanes 1and 13 stands for positive PCR results for healthy control subjects (GIII), lanes 2, 4, 5 and 6 are positive PCR assay results for patients from GI before periodontal therapy, lanes 3, 7 and 8 represents positive PCR results for patients from GI after periodontal therapy, lanes 9, 10 and 12 represents a positive PCR assay results for patient in GII before periodontal therapy, lane 11 represent a positive PCR assay results for patient from GII after periodontal therapy. (b) lanes 1, 3, 5 and 6 represent a positive PCR assay results for patients from GI before periodontal therapy, lanes 2 and 4 represents the positive PCR assay results for GI patients after periodontal therapy. M lane is DNA size marker, A lane is a positive control and B lane is a negative control,