Figure 3. Changes in target gene expression between MycWT and BL associated mutants correspond with changes in H4 acetylation, but not Myc promoter occupancy.

A) Validation of expression microarrays was performed by RT-PCR on Rat1a engineered cell lines expressing indicated form of Myc or vector, to confirm expression levels of known Myc target genes HSP60, Nucleolin, CAD, NME1. The differential regulation of these target genes is consistent with relative expression levels determined by expression microarray. B) ChIP assays using anti-Myc antibodies were performed to determine Myc binding to E boxes in the promoters of indicated target gene C) ChIP assay using anti-acetylated H4 antibodies were performed at the same sites to analyze histone acetylation by the indicated Myc protein. Error bars indicate SD over three biological replicates. Asterisk indicates significant difference compared to MycWT; student’s t-test, *p≤0.05, **p≤0.01,***p≤0.001.