Extended Data Table 1. No evidence for admixture related to sub-Saharan Africans in Natufians.

We computed the statistic f₄(Natufian, Other Ancient; African, Chimp) varying African to be Mbuti, Yoruba, Ju_hoan_North, or the ancient Mota individual. Gene flow between Natufians and African populations would be expected to bias these statistics positive. However, we find most of them to be negative in sign and all of them to be non-significant (|Z|<3), providing no evidence that Natufians differ from other ancient samples with respect to African populations.

Other Ancient	African	f4(Natufian, Other Ancient; African, Chimp)	z	Number of SNPs
EHG	Mbuti	−0.00044	−1.0	254033
EHG	Yoruba	0.00029	0.7	254033
EHG	Ju_hoan_North	−0.00015	−0.4	254033
EHG	Mota	−0.00022	−0.4	253986
WHG	Mbuti	−0.00067	−1.7	261514
WHG	Yoruba	−0.00045	−1.1	261514
WHG	Ju_hoan_North	−0.00046	−1.2	261514
WHG	Mota	−0.00129	−2.3	261461
SHG	Mbuti	−0.00076	−2.0	255686
SHG	Yoruba	−0.00039	−1.0	255686
SHG	Ju_hoan_North	−0.00052	−1.4	255686
SHG	Mota	−0.00091	−1.7	255641
Switzerland_HG	Mbuti	−0.00018	−0.4	261322
Switzerland_HG	Yoruba	0.00019	0.4	261322
Switzerland_HG	Ju_hoan_North	0.00009	0.2	261322
Switzerland_HG	Mota	−0.00062	−0.9	261276
Kostenki14	Mbuti	0.00034	0.7	246765
Kostenki14	Yoruba	0.00120	2.3	246765
Kostenki14	Ju_hoan_North	0.00069	1.4	246765
Kostenki14	Mota	0.00036	0.5	246719
MA1	Mbuti	−0.00038	−0.7	191819
MA1	Yoruba	0.00009	0.2	191819
MA1	Ju_hoan_North	−0.00010	−0.2	191819
MA1	Mota	−0.00038	−0.5	191782
CHG	Mbuti	−0.00051	−1.2	261505
CHG	Yoruba	−0.00012	−0.3	261505
CHG	Ju_hoan_North	−0.00013	−0.3	261505
CHG	Mota	−0.00042	−0.7	261456
Iran_N	Mbuti	−0.00018	−0.4	232927
Iran_N	Yoruba	0.00036	0.8	232927
Iran_N	Ju_hoan_North	0.00041	0.9	232927
Iran_N	Mota	0.00006	0.1	232880