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. Author manuscript; available in PMC: 2017 Feb 25.
Published in final edited form as: Nature. 2016 Aug 25;536(7617):474–478. doi: 10.1038/nature18952

Figure 1. Production and immunogenicity of DNA vaccines.

Figure 1

(a) Schema of ZIKV prM-Env immunogens and deletion mutants. (b) Western blot of transgene expression from (1) prM-Env, (2) prM-Env.dTM, (3) prM-Env.dStem, (4) Env, (5) Env.dTM, (6) Env.dStem, and (7) sham DNA vaccines transfected in 293T cells. Balb/c mice (N=5/group) received a single immunization with 50 µg of these DNA vaccines by the i.m. route. (c) Humoral immune responses were assessed at week 3 following vaccination by Env-specific ELISA. Red bars reflect medians. Cellular immune responses were assessed by (d) IFN-γ ELISPOT assays and (e) multiparameter intracellular cytokine staining assays. Error bars reflect s.e.m.