Table 3.

Summary of calibration curve standards

Analyte	Nominal Conc (ng/mL)	Mean Calculated Conc (ng/mL) n=3	%CV	% Accuracy	r2
Tenofovir	10	10.51	0.40	105.07	>0.9976
	20	18.18	1.45	90.91
	100	98.22	2.15	98.22
	500	527.21	3.87	105.44
	1000	1006.11	1.70	100.61
	2000	1980.62	2.41	99.03
	3000	2997.99	1.28	99.93
	4000	4017.70	1.77	100.44
Emtricitabine	5	4.96	0.20	99.18	>0.9997
	10	10.22	0.21	102.18
	50	49.16	1.75	98.31
	250	248.42	0.68	99.37
	500	507.28	0.36	101.46
	1000	1015.74	2.10	101.57
	1500	1485.11	0.58	99.01
	2000	2013.21	0.93	100.66
Dolutegravir	5	5.00	0.53	99.94	>0.9996
	10	9.97	1.56	99.73
	50	50.20	2.02	100.41
	250	255.86	0.82	102.34
	500	512.29	1.73	102.46
	1000	973.88	1.28	97.39
	1500	1470.73	1.72	98.05
	2000	2003.94	0.81	100.20
Elvitegravir	5	5.09	2.14	101.86	>0.9988
	10	9.72	4.34	97.20
	50	48.70	2.76	97.40
	250	251.53	0.55	100.61
	500	495.93	0.93	99.19
	1000	978.86	2.45	97.89
	1500	1578.63	0.23	105.24
	2000	2033.91	1.03	101.70
Rilpivirine	5	5.03	0.77	100.52	>0.9998
	10	9.87	1.69	98.69
	50	50.21	1.58	100.42
	250	253.88	1.37	101.55
	500	503.43	0.72	100.69
	1000	999.82	2.16	99.98
	1500	1491.50	0.74	99.43
	2000	1982.29	2.35	99.11

%CV, percent coefficient of variation; %RE, percent relative error