Fig. 3. Characterization of system to measure VIC contractility.
A. Completed system at day 0, with hydrogel+VIC (left). F-actin (green), αSMA (red), and nuclei (blue) of hydrogel+VIC after 24 hours of culture in control media (right). B. Cell viability in control and osteogenic conditions over 14 days of culture in the system. C. Cell density in control and osteogenic conditions over 14 days of culture in the system. N ≥ 3. * indicates p < 0.05 versus day 1 control. Error bars indicate SEM.