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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Autoimmun. 2016 Jun 8;73:30–41. doi: 10.1016/j.jaut.2016.05.014

Figure 2. Binding of cord blood IgM to apoptotic cells.

Figure 2

IgM binding to 7AAD(+) Annexin V(+) apoptotic murine thymocytes (A), or freshly isolated 7AAD(−) Annexin V(−) healthy thymocytes (B), in four representative cord blood samples at 1:100 dilution compared to human monoclonal IgM isotype control (Iso IgM). Dose-dependent binding of cord blood IgM (C) or adult serum IgM (D) to 7AAD (+) Annexin V (+) apoptotic thymocytes. Studies were performed by multiparameter flow cytometry with values for geometric mean fluorescence intensity (MFI) depicted. Results are representative of surveys of 8 cord blood and 8 unrelated healthy adult samples, performed in duplicate.