Table 1.
Bacterial strains and plasmids used in this study
| Strains | Description | Source |
|---|---|---|
| S.mutans UA159 | Parent strain | (Liu et al., 2011) |
| ΔpdeA | PdeA deletion; Kanr | This study |
| ΔpdeA /pdeA | PdeA complementation; Kanr; Ermr | This study |
| ΔcabPA | CabPA deletion; Ermr | This study |
| ΔcabPB | CabPB deletion; Ermr | This study |
| ΔpdeAΔcabPA | PdeA and CabPA double mutant; Kanr; Ermr | This study |
| ΔpdeAΔcabPB | PdeA and CabPB double mutant; Kanr; Ermr | This study |
| ΔpdeAΔgtfB | GtfB insertion mutant on PdeA; Kanr; Specr | This study |
| ΔgtfB | GtfB deletion; Kanr | This study |
| ΔcabPA/tap | CabPA deletion with tap; Kanr; Ermr | This study |
| ΔcabPA/tap:cabPA | Recombinant CabPA with tap; Kanr; Ermr | This study |
| ΔpdeAΔgtfB/gtfB | Complementation of PdeA-GtfB using GtfB; Ermr ;Kanr; Specr |
This study |
| WT/DAC | Overexpressed DAC on wild type S. mutans | This study |
| S. mutans/gfp | gfp-tagged S. mutans UA159; Kanr | (Liu et al., 2011) |
| ΔpdeA/gfp | gfp-tagged PdeA deletion; Kanr; Ermr | This study |
| ΔpdeAΔgtfB/gfp | gfp-tagged PdeA-GtfB double mutant; Kanr; Ermr |
This study |
| ΔgtfB/gfp | gfp-tagged GtfB deletion; Kanr; Ermr | This study |
|
E.coli pET28b–CabPA |
CabPA expression; Kanr | This study |
|
E.coli pET28b–CabPB |
CabPB expression; Kanr | This study |
|
E.coli pET28a–sumo-VicR |
VicR purification; Kanr | Wu Lab |
| E. coli Top 10 | Cloning strain | Invitrogen |
| E. coli BL-21 | Protein production strain | Invitrogen |
| Plasmids | ||
| pVPT | E. coli-Streptococci shuttle vector; Ermr | (Zhou et al., 2008) |
| pVPT-pdeA | pVPT carrying PdeA ORF; Ermr | This study |
| pVPT-tap | pVPT carrying tap tag; Ermr | Wu Lab |
| pVPT-tap-cabPA | pVPT carrying tap tag and CabPA; Ermr | This study |
| pET28b(+) | Expression plasmid; Kanr | Novagen |
| pET28a–sumo | Expression plasmid; Kanr | Wu Lab |
| pET28b–cabPA | pET28b(+) carrying cabPA ORF; Kanr | This study |
| pET28b–cabPB | pET28b(+) carrying cabPB ORF; Kanr | This study |