Table 2.

Primers used in this study

Primers	Sequence (5’-3’)	Description
pdeAupF	TATGATATTGTCCTTCCTCATC	PdeA deletion
pdeAupR	TATTCTCATTTTAGCCATTATCTAAACCTCTTAAGCCCTA	PdeA deletion
pdeAdnF	CTGGATGAATTGTTTTAGGATGAAAGTTATTTTTTTAGCA	PdeA deletion
pdeAdnR	CGAAATCCACTACGATTACT	PdeA deletion
pedAcheckF	ATGTGGTTCATTCGATTAAA	Check PdeA mutant
pdeAcheckR	GAACTTCTAAATTTTCATAATTGAC	Check PdeA mutant
pdeAF	ATGAAAAGATTTCGTTTTGCCAC	Amplify PdeA
pdeAR	TTAAGCTTCCTCGTTTCCTG	Amplify PdeA
kanF	ATGGCTAAAATGAGAATA	Amplify kanamycin
kanR	CTAAAACAATTCATCCAG	Amplify kanamycin
ermF	GGAAGGAGTGATTACATGAA	Amplify rythromycin
ermR	GAAGCTGTCAGTAGTATACC	Amplify rythromycin
cabPAupF	GCCTTATCAGATGAAGTTGTC	CabPA deletion
cabPAupR	TTCATGTAATCACTCCTTCCAGCTACCCTCCTTTTGATAAC	CabPA deletion
cabPAdnF	GGTATACTACTGACAGCTTCGGAGGAAGAAGCTATGAAAAT	CabPA deletion
cabPAdnR	CTACTATCGAGCTCTTCAACTG	CabPA deletion
cabPAcheckF	AAGATATGTCAGATGATGACTTGA	Check cabPA mutant
cabPAcheckR	AGCTGGACTTTTCTAATTCTTTC	Check cabPA mutant
cabPBupF	CCACAGCATAAGTATGACTAA	CabPB deletion
cabPBupR	TTCATGTAATCACTCCTTCC GATTTACCTCCGAATTATAA	CabPB deletion
cabPBdnF	GGTATACTACTGACAGCTTCGCTATTATGAATAAAAGTATGG	CabPB deletion
cabPBdnR	TAAACTGAGAAAAGAGCGGCC	CabPB deletion
cabPBcheckF	TTTTTGTAATACTTTTAACGCATAT	Check cabPB mutant
cabPBcheckR	ACCAAGAAACATCAGTAGAAGC	Check cabPB mutant
cabPAF	GTCAGGATCCG ATGAAAATTATTATTGTCGGATGTGG	CabPA expression
cabPAR	GCCGGAGCTC TCAGAAAATAAAGCGTTCTCTC	CabPA expression
cabPBF	GTCAGGATCCG ATGAAAATTATTGTCGTTGGTGG	CabPB expression
cabPBR	GCCGGTCGAC TTACCTCTTTAATAAATCATAAA	CabPB expression
Q16sRNAF	AGCGTTGTCCGGATTTATTG	qRT-PCR
Q16sRNAR	CTACGCATTTCACCGCTACA	qRT-PCR
QgtfBF	CACTATCGGCGGTTACGAAT	qRT-PCR
QgtfBR	CAATTTGGAGCAAGTCAGCA	qRT-PCR
QgtfCF	GATGCTGCAAACTTCGAACA	qRT-PCR
QgtfCR	TATTGACGCTGCGTTTCTTG	qRT-PCR
QgtfDF	TTGACGGTGTTCGTGTTGAT	qRT-PCR
QgtfDR	AAAGCGATAGGCGCAGTTTA	qRT-PCR
cabPAtapF	GCCGGTCGACATGAAAATTATTATTGTCGGATGTGG	TAP construct
cabPAtapR	GCGCGGTACCTCAGAAAATAAAGCGTTCTCTC	TAP construct