Fig. 1.

Generation of lin⁻CD34⁺CD43⁺CD45⁺ primitive hematopoietic cells from CML-iPSCs. (a) Schematic diagram of hematopoietic differentiation from iPSCs. Phenotype and its designation are shown under and on the top of cells, respectively. Prefix i indicates iPSC-derived. FSGm36 = Flt3L, SCF, GM-CSF, IL3, IL6. (b) Phenotypes of CD45⁺ cells obtained from CML-iPSCs after differentiation on OP9 (day 9) and following their expansion/differentiation with cytokines in stroma-free cultures (as indicated in A). Plots show isotype control (open) and specific antibody (shaded) histograms. Representative results from three to four independent experiments are shown. (c) and (d) Rhodamine (Rho) efflux and ALDH activity in isolated CML iCD34⁺ cells. Representative dot plots show Rho efflux and ALDH activity in CD34⁺CD38⁺ and CD34⁺CD38⁻ populations (c). Graph shows quantification of Rho efflux (di) and ALDH assay (dii). The values are mean ± SEM of % of Rho^low cells (% of Rho^low_verapamil− – Rho^low_verapamil+) and ALDH⁺ cells (% of ALDH⁺_DEAB− – ALDH⁺_DEAB+) from three experiments respectively. DEAB = diethylaminobenzaldehyde, ALDH = aldehyde dehydrogenase. (e) Summary of findings.