Figure 6.

Inhibitory effects of MD-1 on DMN-induced hepatic fibrosis in rats. (A) The sectioned rat liver tissues were stained with Van Gieson staining reagent to show the proliferated collagen fibers (stained with red) segmented and surrounded pseudolobules in the positive control group, and MD-1 treatment significantly reduced the collagen fibers. Original magnification: 200×. (B) Top: Expression of p-EGFR, p-AKT, α-SMA, cyclin D1, and p-Smad in sectioned rat liver was examined by immunohistochemistry. The percentages of positive cells (stained with brown) were counted within five fields of view for each section using a 20× objective lens. Original magnification: 100×. Bottom: Statistics for p-EGFR, p-AKT, α-SMA, cyclin D1, and p-Smad. *P < 0.05; **P < 0.01; ***P < 0.001. (C) Top: Detection of serum ALT and AST levels on fully automated biochemical analyzer. Bottom: Detection of collagen I by ELISA. *P < 0.05; **P < 0.01; *** P < 0.001