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. 2016 Aug 3;7(9):624–637. doi: 10.1007/s13238-016-0300-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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The HMF-exposed primary NSs can differentiate into astrocytes/glia cells and neurons. HMF-exposed and GMF primary NSs from P2 mice (day 7) were collected and induced to differentiate under the GMF condition for 5 days. Representative differentiated large (A–H) and middle (A’–H’) size spheres from the GMF (A–D/A’–D’) and HMF (E–H/E’–H’) group show NS cells from HMF condition could differentiate into both neuron (ßIII-tubulin, green) and glial cells (GFAP, red), compared to those from the GMF group. Nuclei were stained with Hoechst (blue)