Fig. 4.

Assessment of cell death related proteins. After 12-h incubation, protein was extracted from the cells and western blot analysis was performed to detect the expressions of diverse proteins. a Changes in expressions of cell death related proteins such as caspase-3, Bcl-2, HSP 70, and cleaved caspase-8 were detected by western blot analysis. b Changes in expressions of MAPK pathway-related proteins such as p-JNK, JNK, p-p38, and p38 were detected by western blot analysis. c Changes in expression of LC3 was detected by western blot analysis