Fig. 3.

Changes in binding of the transcription factor NFATc4 to a DNA-binding element designed for the NFAT consensus sequence in the skeletal muscle of I. tridecemlineatus over the torpor-arousal cycle. DNA-protein interaction (DPI)-ELISA absorbance readings were corrected by subtraction of negative controls containing no protein, and values were expressed relative to EC. Histograms show mean relative values ± S.E.M., n = 4 independent biological replicates for each of the six experimental conditions. Data was analyzed using a one-way analysis of variance with a post hoc Tukey’s test (p < 0.05); for each parameter measured, values that are not statistically different from each other share the same letter notation