Fig. 4.

Effect of adjusting temperature on transcription factor-DNA binding of NFATc1, c3, and c4. a) Transcription factor-DNA binding was measured at 37, 24 (room temperature), and 4 °C at the EC sampling point before hibernation is initiated. b) Transcription factor-DNA binding was measured at 37, 24 (room temperature), and 4 °C at the LT sampling point deep within hibernation. c) Changes in the binding of NFAT c1, c3, and c4 transcription factors to DNA at physiological temperatures from EC (37 °C) and LT (4 °C). Modified DPI-ELISA absorbance readings were corrected by subtraction of negative controls containing no protein, and values were expressed relative to 37 °C for both EC and LT. Data in panel (a) and (b) were analyzed using a one-way analysis of variance with a post hoc Tukey’s test (p < 0.05), for each parameter measured, and the data in panel (c) were analyzed using a paired T test (p < 0.05). Values that are not statistically different from each other share the same letter notation