Figure 1.

UBQLN2 Is Required for Cell Survival after Heat Shock

(A) Schematic of the known domains of UBQLN2, their binding partners, and reported familial disease mutations shown in italics.

(B) Binding partners of UBQLN2 that were identified by immunoprecipitation (IP) of UBQLN2 from mouse brain lysate followed by mass spectrometry.

(C) Depletion of UBQLN2 by two independent siRNAs (72 hr) leads to cell death on heat stress.

(D–F) UBQLN2, HSP70, and proteasome, but not UBQLN1 or UBQLN4, co-purify with insoluble ubiquitin-rich aggregates upon heat stress.

(G–I) UBQLN2 inducibly interacts with proteasomes, ubiquitylated proteins, and HSP70 after heat shock and loses binding to UBQLN1 and UBQLN4.

See also Figures S1 and S7.