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. 2016 Aug 30;7:1376. doi: 10.3389/fmicb.2016.01376

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Copyright © 2016 Espina, García-Gonzalo and Pagán.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fluorescence histogram overlays obtained by flow cytometry measurement of PI-stained E. coli BW25113 cells of untreated cells and after thermal treatments at 53°C. Each overlay represents the frequency distribution of the fluorescence intensity (fluorescence-area values) of a total of 10000 events per sample, corresponding to the untreated sample (lighter overlay), or samples treated for 2, 4, 6, 10, 16, or 20 (darker overlay) min. Results shown from a representative experiment repeated three times with similar results.