Table 1.
Fungi isolates used in the conventional PCR and LAMP assay.
| Speciesa | RAPD groupb | Origin |
PCR productc |
||
|---|---|---|---|---|---|
| Host | Geographical | Conventional PCR | LAMP | ||
| Didymella bryoniae (DBJSJY2) | RGI | Muskmelon | Jiangsu | + | + |
| Didymella bryoniae (DBAHHF2) | RGI | Muskmelon | Anhui | + | + |
| Didymella bryoniae (DBZJNB5) | RGI | Muskmelon | Zhejiang | + | + |
| Didymella bryoniae (DBJSNJ60) | RGI | Watermelon | Jiangsu | + | + |
| Didymella bryoniae (DBZJNB7) | RGII | Watermelon | Jiangsu | + | + |
| Ascochyta pinodes ZJ-1 | – | Pea | Zhejiang | - | - |
| Colletotrichum orbiculare NJ-1 | – | Watermelon | Jiangsu | - | - |
| Pythium paroecandrum Drechsler | – | Gourd | Jiangsu | - | - |
| Alternaria alternata LH1401 | – | Muskmelon | Jiangsu | - | - |
| Fusarium verticillioide | – | Gourd | Jiangsu | - | - |
| Fusarium oxysporum f.sp. niveum Race 0 | – | Watermelon | Jiangsu | - | - |
| Fusarium oxysporum f.sp. niveum Race 1 | – | Watermelon | Jiangsu | - | - |
| Fusarium oxysporum f.sp. niveum Race 2 | – | Watermelon | Jiangsu | - | - |
aAll isolates of fungi species were maintained in the collection of Jiangsu Academy of Agricultural Sciences.
bBased on the presence of a PCR product of the expected size for RAPD group assignments (RGI and RGII) using RG-specific SCAR primers (Supplementary Figure S1; Keinath et al., 2001; Babu et al., 2015).
cNote that presence (+) or absence (-) are based on the presence of a PCR or LAMP product of the expected size.