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. 2016 Aug 30;7:1317. doi: 10.3389/fpls.2016.01317

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Copyright © 2016 Li, Gou, Chen, Li and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Phylogenetic analysis of the putative CYP71 candidates of this study with the previously published known GAA or GAA-derived oxidizing enzymes. Amino acid sequences were aligned using Clustal X software. The tree was inferred by the neighbor-joining method using MEGA 6.0 software. The scale bar represents 0.2 amino acid substitutions per site. Numbers indicate the bootstrap values of 1000 replicates. Germacrene A oxidase (Helianthus annuus), ADF43082.1; germacrene A oxidase (Tanacetum parthenium), AHN62855.1; germacrene A oxidase (Saussurea costus), ADF43081.1; germacrene A oxidase (Cynara cardunculus var. altilis), AIA09036.1; germacrene A oxidase (Lactuca sativa), ADF32078.1; germacrene A oxidase (Cichorium intybus), ADF43080.1; germacrene A oxidase (Barnadesia spinosa), ADF43083.1; costunolide synthase (Lactuca sativa), AEI59780.1; costunolide synthase (Tanacetum parthenium), AGO03790.1; germacrene A acid 8-beta-hydroxylase (Helianthus annuus), F8S1H3.1; parthenolide synthase (Helianthus annuus), AHM24033.1.