Table 2.
The evaluation of the sequencing depth of the X. strumarium transcriptome by monitoring unigenes encoding the conserved mevalonate (MVA) pathway enzymes.
| The names of the MVA pathway enzymes | ID numbers of the query sequences | The highest hit in the X. strumarium transcriptome | Sequencing depth | Length (amino acid) |
|---|---|---|---|---|
| AACT | AAM00280.1 | CL7008.Contig2_All (79%) | 1741.2526 | 409 |
| HMGS | AAD00297.1 | CL4694.Contig1_All (76%) | 649.8398 | 458 |
| HMGR | AAA67317.1 | Unigene4899_All (67%) | 1305.6618 | 613 |
| MVK | AED93690.1 | Unigene5281_All (61%) | 806.5116 | 387 |
| PMK | AES72471.1 | Unigene21560_All (69%) | 81.0194 | 489 |
| MVD | CAA76803.1 | Unigene21721_All (75%) | 784.0556 | 422 |
Genes encoding all the putative MVA pathway enzymes in X. strumarium were identified by a tBLASTn search against the X. strumarium transcriptome using their respective known proteins as the query sequences.
AACT, acetoacetyl-CoA thiolase; HMGS, 3-hydroxy-3-methylglutaryl-CoA synthase; HMGR, 3-hydroxy-3-methylglutaryl-CoA reductase; MVK, mevalonate kinase; PMK, phospho mevalonate kinase; MVD, mevalonate diphosphate decarboxylase.