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. 2016 Aug 30;7:326. doi: 10.3389/fimmu.2016.00326

Figure 1.

Figure 1

Timeline of co-culture experiments. In the case of intracellular FACS requiring addition of BrefeldinA, supernatant was taken before addition of BrefeldinA or from replicate cultures without BrefeldinA. Melanoma cell lines were thawed at least 1 week prior to the experiment.