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CTL-mediated killing of melanoma cell lines in co-cultures is antigen-specific. Three different MelanA-specific CTL:melanoma cell ratios are shown in green. Yellow-fever-virus- or Epstein–Barr-virus-specific CTLs did not kill the melanoma cells (blue). The number of living cells was measured by flow cytometry. N = 2, except for T1185B 1:3 with MelanA-specific CTLs and 3:1 with neg. control CTLs N = 4. Error bars indicate SD.
