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Co-cultured antigen-specific CTLs produce cytokines. MelanA-specific CTLs accumulate intracellular IFNγ and TNFα with strongest expression at 6 h of co-culture with the melanoma cell lines Me275 (top row) and Me290 (bottom row). Non-specific CTLs did not accumulate intracellular cytokines. Cytokine expression was measured by flow cytometry after intracellular staining of the co-cultured cells. N = 2, error bars indicate SD.
