FIGURE 2.

Organ-specific expression of Arabidopsis Kunitz-PI;1. (A,B) Kunitz-PI;1 transcript (A) and Kunitz-PI;1 protein (B) abundance in flowers, in leaves of 3-week-old plants, and in 3-days old etiolated seedlings. The gel in (A) shows RT-PCR products visualized by ethidium bromide staining. Actin transcript levels were analyzed as internal constitutive marker. For SDS-PAGE (B), 50 μg of total protein was loaded per lane. Replicate gels were used for immunodetection using Kunitz-PI;1-specific antibodies (upper panel) and Coomassie staining (lower panel). Note that (B) is a composite diagram.