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. 2016 Aug 30;7:1246. doi: 10.3389/fpls.2016.01246

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Copyright © 2016 Boex-Fontvieille, Rustgi, von Wettstein, Pollmann, Reinbothe and Reinbothe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Immunolocalization of Arabidopsis Kunitz-PI;1 protein. Seedlings were grown for 3 days on ACC-containing medium. Tissue sections from the basal part of the hypocotyls (A,E) and apical part of the hypocotyls (B,F), the apical hook (C,G), the cotyledons (D,H), and a longitudinal section of the upper half of a seedling (I) were incubated with Kunitz-PI;1-specific antibodies (A–D,I) or preimmune serum (E–H). Detection of the antigen-antibody complexes was made using an alkaline phosphatase-based assay with NBT and BCIP.