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. 2016 Aug 30;7:1246. doi: 10.3389/fpls.2016.01246

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Copyright © 2016 Boex-Fontvieille, Rustgi, von Wettstein, Pollmann, Reinbothe and Reinbothe.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Kunitz-PI;1 promoter activity in response to wounding. (A,B) The hypocotyls of 3-days old etiolated seedlings were cut into two halves of which the aerial part comprising the apical hook and cotyledons (A) and the basal part comprising the root (B) were analyzed further by GUS staining. Red and black arrows mark regions of high and low Kunitz-PI;1 promoter activity, respectively. (C–E) Etiolated seedlings were wounded by crushing the hypocotyls with tweezers near the apical hook region (red arrows) and GUS staining made 24 h thereafter (D,E). (C) Unwounded control. Size markers are indicated.